MaestroGen Protein Ladders, comprising of fragments of specific base pair length, are available for estimation of molecular weights from 8 to 245 kilodalton (kDa).
02101-250 AccuRuler RGB Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights from 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-polyacrylamide gel electrophoresis (SDSPAGE) with Tris-glycine-SDS running buffer.
02101-250 AccuRuler RGB Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, for verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose), and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. It is unnecessary to heat, dilute, or add reducing agent before loading.
Two reference bands
Red at ~75 kDa and green at ~25 kDa
Ready to use
No need to boil
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively
General Western transferring
2 μl to 3 μl per well for general Western transferring
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