Brand
Maestrogen
MaestroGen DNA Ladders, comprising of fragments of specific base pair length, are available for estimation of molecular weights from 100 bp to 10 Kb. For easy determination of band size, several DNA Ladders include bands that are two to three times more intense.
02004-500 AccuRuler 1 Kb Plus DNA ready-to-use Ladder is suitable for sizing linear double stranded DNA fragments from 100 bp to 10 Kb. A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and PCR products to yield 13 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The 1 Kb and 3 Kb bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (assuming a 0.5 μg / 5 μl load) for approximating the mass of DNA in comparably intense samples of similar size.
Accuracy
Accurate size and mass estimations
Bands Increased Intensity
1 Kb and 3 Kb bands have increaesd intensity
RTU
Ready to use
Source
PCR products and double-stranded DNA digested with appropriate restriction
enzymes, are phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0)
and 10mM EDTA
| Cat. No. | 02004-500 |
| Size | 50 μg / 500 μl |
| Range | 100 bp to 10 Kb |
| Bands Increased Intensity | 1 Kb and 3 Kb bands |
| Number of Bands | 13 |
| Concentration | 114 μg / ml |
| Recommended Load | 5 μl per well |
| Mass/Band | 0.5 μg / 5 μl |
Storage & Loading Buffer | 10 mM Tris-HCl (pH 8), 10 mM EDTA, bromophenol blue, xylene cyanol |
| FF and glycerol | |
Storage | 24 months at -20°C |
| 12 months at 4°C | |
| 6 months at 25°C |
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