MaestroSafeTM Nucleic Acid Prestained Dye represent a new and safe class of nucleic acid dye for visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose gels. The dye is developed to replace toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis for visualization of nucleic acids in agarose gels.
MaestroSafeTM Nucleic Acid Prestained Dye is non-carcinogenic by the Ames-test. The results are negative in both the mouse marrow chromophilous erythrocyte micronucleus and mouse spermary spermatocyte chromosomal aberration tests.
With MaestroSafeTM Nucleic Acid Prestained Dye, you do not need to do post-stain or pre-mix with agarose gel. All you have to do is mix the dye with agarose in process of preparation and run electrophoresis normally. After the electrophoresis, view and document your results as you do with EtBr staining protocols.
MaestroSafeTM Nucleic Acid Prestained Dye emits green fluorescence for dsDNA or ssDNA, and red fluorescence for RNA. This dye has one excitation (470 nm) and two emission spectra (520 nm and 635 nm).
According to the cloning efficiency test, DNA stained with EtBr and exposed by UV light will reduce the efficiency of cloning. The combination of MaestroSafeTM and LED light shows a better cloning efficiency.
|Description||Prestained with agarose|
|Storage||12 months at 4°C|
|For laboratory only. Not for clinical applications.|
|1. Prepare a 100ml agarose solution.|
|2. Heat solution until particles disappeared.|
|3. Add 1.5-2μl MaestroSafe TM Nucleic Acid Prestained Dye into solution and mix gently.|
|4. Let the solution cool down to 60-70℃ and cast the gel.|
|5. Load samples into well and perform electrophoresis.|
|6. View the results under traditional UV light after electrophoresis.|
|7. Or use MaestroGen UltraSlim or UltraBright LED Transilluminator for not only good performance but health concerns|
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