The Tristar 3 Multimode Microplate Reader provides you with application flexibility for today, tomorrow, and beyond to master your changing plate reading applications:
Our patented ONE-4-ALL optics have been optimised to combine the stability and user-friendliness of a multimodal optical system with the sensitivity and versatility of dedicated optical devices.
ONE-4-ALL optics work for both filter- and monochromator-based applications, so there´s never a compromise in performance in any mode.
The quick-change filter technology of the Tristar series provides you with the flexibility required to meet your application needs: up to 40 different excitation and emission filters can be easily mounted on exchangeable filter slides.
The filters are characterized by high transmissions properties which can be up to 25-fold that of monochromators. Technologies like Time-Resolved Fluorescence (TRF) can be measured more efficiently with filters.
Furthermore, filters with a large bandwidth are available to analyze fluorophores with wide spectra and all luminescence-based assay requiring to use filters, e.g. BRET, BRET2 and NanoBRET™ assays.
The ergonomic design of the system provides full front access for all key operations (e.g. plate loading, filter change and connecting reagents). Reagent vials can be stored in an integrated front compartment, providing easy access and visibility. It contains a removable trough that can be filled with water or ice to keep all reagents cooled. Different reagent tube holders are available to ensure secure handling of valuable reagents. The system has a flat surface on its top, providing enough space to put down a laptop.
Do you often see a few high-intensity samples surrounded by wells of low-intensity when running your samples? Then you´re potentially affected by a phenomenon called crosstalk, that can produce wrong results and, hence, wrong conclusions. The crosstalk reduction design of the Tristar 3 does almost eliminate this problem: with a crosstalk of 10-6, you never have to worry about the position of your high-intensity samples in your plate.
The Tristar 3 can be equipped with up to 3 JET injectors with variable volumes in pre-measurement or measurement position. The ultra-fast injection speed of JET injectors eliminates any delay between injection and measurement and enables measurement of fast reactions, e.g. flash luminescence.
Berthold Technologies’ JET injectors use Teflon bellows for accurate and fast injections and guarantee most efficient mixing as well as extreme longevity.
Choose between different software options according to your application needs:
ICE software is an intuitive, wizard-guided software that walks you through the entire process of setting up your system, collecting your data and reporting the results. The software package is as flexible as your research thanks to the many setting options and freely configurable combinations of operating sequences.
The MikroWin software is a complete software solution providing instrument control, data collection, analysis and reporting. The software is available in different versions, reflecting different user needs. The complete Advance version of the software provides 21 CFR Part 11 compliance and additional QC features like audit trail or configurable user hierarchies.
For many laboratories, validation, qualification and ensuring compliance with a number of GMP and GLP requirements is essential. We provide a number of tools and services to help you ensure that your system is running at peak performance.
We provide a variety of tools to help you check and confirm the performance of your products over time.
Berthold Expert Services provide a team of dedicated and factory-trained engineers and experts to optimize your productivity. We and our local partners are always at your disposal.
Our team of technical support scientists is your partner to overcome the unique challenges your application brings. Contact our team to discuss your assay or experiment design, data analysis questions, troubleshooting and more.
|Detectors||Low-noise photomultiplier tube that can operate in single photon counting mode and in our advanced Pulse Height Detection mode|
|Photodiode for absorbance|
|Measurement modes||Endpoint, Kinetics, Repeated, Spectral scan, Area scan (up to 100 x 100 points per well)|
|Filters||High quality interference filters|
|Filter handling||Easy to change filter slides with up to 5 filters per slide, additional slides available|
|Optical system||ONE-4-ALL optics|
|Light source||Xenon flash lamp|
|Spectral range Absorbance||200-1000 nm|
|Spectral range Luminescence||Standard: 280-650 nm|
|FL models: 280-850 nm|
|Spectral range Fluorescence||Excitation: 200-1000 nm|
|Emission, standard: 280-650 nm|
|Emission, FL models: 280-850 nm|
|Injectors||Up to 3 JET injectors with frictionless operation|
|Injector volume||10-100 µL in 1 µL steps|
|Injection speed||200 – 440 μL/sec|
|Injector accuracy||Better than 2% across the entire volume range|
|Injector precision||Better than 2% across the entire volume range|
|Microplate formats||6- to 384-well|
|Plate height adjustment||3 different frames depending on plate type|
|Additional sample formats (optional)||µDrop plate for microvolumes|
|Adapter for cuvettes|
|Adapter for petri dishes|
|Adapter for Terasaki plates|
|Shaking||Linear, orbital, double orbital, with selectable speed and diameter|
|Temperature control||From ambient +5° to 45° C, includes additional cooling for the photomultiplier|
|Gas connection||Yes (optional)|
|Absorbance precision||Better than 0.6%|
|Absorbance accuracy||Better than 2%|
|Luminescence sensitivity||<6 amol (0.03 pM) ATP/well (96-well plate)|
|Fluorescence sensitivity||<7 amol Fluorescein/well (384-well small volume plate)|
|Crosstalk||10-6 (black plates)|
|Dynamic range||>6 orders of magnitude|
|Interface||Windows software, connected to computer via USB|
|Operating system||Windows 10|
|Available software packages||ICE Software: wizard-guided operation, includes ratio calculation and display of kinetic curves|
|Optional: Mikrowin for advanced curve fitting data evaluation, easy automation and compliance with CFR 21 part 11.|
|Power Supply||110-240 VAC ± 10%|
|Operating Voltage||24 VDC ± 5%|
|Power consumption||140 VA|
|Temperature tolerance||Storage: 0-40° C|
|Operation: 15-35° C|
|Humidity tolerance||10-80% non-condensing|
|maximum relative humidity of 80% for temperatures up to 31°C|
|decreasing linearly to 50% relative humidity up to 40°C|
|Altitude||max 2000 m (above sea level)|
|Dimensions (W x D x H)||391 x 470 x 344 mm|
|Weight||approx. 20 kg|
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