Sentra Biosains Dinamika

Berthold - Crocodile 5 in 1 Workstation

Catalog No. 84024-01 Category


Running your ELISA assays without automation requires an hour or more of your attention, making it difficult to plan other important activities while your assay is being processed. Crocodile 5-in-one provides the same functionality as five individual instruments in a footprint similar to a stand-alone-reader. Using Crocodile will reduce assay times by eliminating the need to move plates between dispenser, washer, incubator, shaker and reader. This allows you to perform other important activities.

Forget about the huge workstations you have seen in large hospitals. Crocodile is only 26.5 cm wide. With all reagent bottles placed to the left of the instrument, it requires no more than 40 cm of space in width.

Crocodile 5-in-one provides the same functionality as five individual instruments but requires the bench space only of a typical ELISA reader:

  1. Dispenser
  2. Shaker
  3. Incubator
  4. Washer
  5. Reader

Total flexibility

Crocodile will adapt to any 96 well assay. Crocodile’s strength is its versatility. Not only can it be used as a powerful and flexible assay processor, offering unattended performance, its functions also can be used to replace individual devices such as plate washers and plate readers. Unlike other ELISA workstations in the market, the Crocodile can perform assays that require to seal the plate during some incubations: just insert a manual step in the protocol and the instrument will play a sound to warn the operator, and instruct him/her to put or remove the film. And, of course, you also have total flexibility in wavelength choice: in addition to the preinstalled filters for ELISA (405, 450, 492 and 620 nm), 4 positions are available for custom filters.

Plug and play

All functional modules are housed in one compact box. Simply connect the unit with the power outlet and a notebook or desktop computer and you are all set for analysis. The tubing for wash and waste liquids is preinstalled, ready to be introduced into the wash and waste bottles. In everyday use, preparing to use Crocodile won´t give you any headaches. Wash, waste, and reagent bottles are readily accessible, right next to the instrument. The instrument is ready for start-up within minutes.

Validation made easy

The performance validation of the Crocodile is supported by our 3Q Package: Installation Qualification, Operation Qualification and Performance Qualification steps are performed according to detailed instructions provided. The results are documented in checklists according to the specific features of the instrument. In addition, we provide an optional Absorbance Test Plate to check the performance of the reader.

Easy maintenance and reliable service

Maintenance is also really simple: flushing of the system and routine replacement of the tubing is made easy by the intuitive design of Crocodile. The instrument is very robust due to the small number of moving parts. In addition, our technical experts are available to help if needed, and our service is available to ensure trouble-free operation over the lifetime of the instrument.

Convenient PC software

The user-friendly software easily adapts to any 96 well assay protocol. The five different functions can be in any sequence, one or several times each (for example one to four dispensing steps or multiple washing steps). Setting of each step is flexible and intuitive. Once you have chosen an assay protocol, the screen will show a graphic of the entire assay schedule, as it will be performed by Crocodile. The optional MikroWin software is available when quantitative and qualitative data analysis is needed.



Sample format1 x 96-well microplate (ANSI SLAS)
Power supplyDesktop power supply and power cord. Input 110-240 V AC, output 24 V/3.3 A DC
Dimensions (W x D x H)263 x 626 x 258 mm
Weight14.8 kg
Operating temperature10-35° C
HumidityUp to 75% (non condensing)


Type4 independent precision pumps
Volume Range10-2000 µL
Precision<2% CV at 100 µL
Accuracy<5% at 100 µL
Reagent supportAny bottle or vial type can be attached
Manifold configuration

Autoclavable 8-way manifold with 2 parallel needles

Dispense volume25-1000 µL
Wash modesStandard, Overflow, Soak, Two-Point, Sweep
Wash buffer capacity3 x 1 L bottles for different wash solutions
Waste liquid container2 L bottle
Temperature rangeFrom ambient +4° C to 55° C
Temperature stability±1° C across plate at 37° C
Incubation timeProgrammable
Temperature monitoringYes
ShakingIndependent linear motion 5-20 Hz
Dynamic range0-3.0 OD at 450 nm
Spectral range400-690 nm
Preinstalled filters405, 450, 492, 620 nm
Total filter slots8
Reading channels8 independent photometric channels plus reference channel

Mono- and bichromatic reading

Precision<1.5% CV (0.01-0.5 OD)

<1% CV (0.5-2.0 OD)

<1.5% CV (2.0-3.0 OD)

Accuracy±0.01 OD or 2.5% (whichever is greater)



Related Products