Sentra Biosains Dinamika

GeneDireX - Total RNA Isolation Kit (Plant)

Catalog No. NA020-0100 Category

Size: 100 Reactions

Sample: 100 mg of fresh plant tissue or 50 mg of dry plant tissue
Format: Spin column
Operation time: within 60 minutes
Elution volume: 50-200 μl
Yield: up to 30 μg

Features

  • Delivers high-quality total RNA with the fast procedure, ready-to-use RNA for high performance in any downstream application.
  • Consistent RNA yield from a small amount of the starting material.

Kit Contents

ContentsSize
Buffer RP110 ml
Buffer W145 ml
Buffer W2 (add ethanol)15 ml (60 ml)
Buffer RE10 ml
RP Column50 pieces x 2
Collection Tube50 pieces x 2

The Total RNA Isolation Kit provides a fast, simple, and cost-effective method for total RNA isolation from plant samples. Detergents and chaotropic salt are used to lyse cells and inactivate RNase. The specialized high-salt buffering system allows RNA species, longer than 100 bases, to bind to the glass fiber matrix of the spin column. The isolated total RNA is suitable for a variety of routine applications including RT-PCR, Northern Blotting, cDNA Synthesis and Mapping. The entire procedure can be completed within 60 minutes.

Applications

  • RT-PCR.
  • Northern blotting.
  • Real-time RT-PCR.

Quality Control
The quality of the Total RNA Isolation Kit (Plant) is tested on a lot-to-lot basis to ensure consistent product quality.

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