Orion II is a state-of-the-art instrument offering complete configuration flexibility. It features high sensitivity thanks to highly efficient optics and photon counting mode. Reagents can be connected easily, and the low dead volume (only 450 µL) saves costly reagents. Orion II can be equipped with up to four reagent injectors and temperature control up to 50°C. Double Injection Design (DID) allows parallel processing of samples and saves valuable assay time.
The Orion II LB 965 meets all currently known requirements for an all-purpose microplate luminometer. The reader supports all basic luminescence technologies including:
The outstanding sensitivity of Orion II is the result of the unique design, advanced engineering, and high-quality workmanship. All functions of the Orion II are seamlessly integrated to render superior, user-friendly performance.
The detector is operated in photon counting mode, which guarantees the lowest signal background for excellent signal to noise ratio and the highest linearity. The plate adjustment mechanism automatically compensates for variations in microplate size. The built-in safety circuit protects the detector from potential damage resulting from accidental exposure to high levels of light. In addition, a warning is produced should a sample ever exceed measurement range.
Do you often see a few high-intensity samples surrounded by wells of low-intensity when running your samples? Then you´re potentially affected by a phenomenon called crosstalk, that can produce wrong results and, hence, wrong conclusions. The special design of the Orion II does almost eliminate this problem: with a crosstalk of 3 x 10-5, you never have to worry about the position of your high-intensity samples in your plate.
The combination of reagent addition options is virtually unlimited. Up to four automatic reagent injectors are available with injection volumes of 10 to 150 μL each. For increased throughput, up to 2 injectors can be installed in measurement position for simultaneous injection. The optimized reagent holders accommodate any shape and any size bottle in a position that allows the complete use of valuable reagent.
The Orion II system has the capability to run samples and process measurements in the most time efficient manner. The proprietary Double Injection Design (DID) allows parallel processing of samples saving valuable assay time. DID has been applied to both 96 well and 384 well configurations.
|Detectors||Low-noise photomultiplier tube in single photon counting mode|
|Measurement modes||Endpoint, Kinetics, Repeated, Batch|
|Spectral range luminescence||300-600 nm|
|Injectors||Up to 4 syringe injectors|
|Injector volume||10-150 µL in 1 µL steps|
|Microplate formats||96- and 384-well|
|Shaking||Linear, orbital, cross shaking, with selectable speed|
|Temperature control||From ambient +5° to 42° C or to 50° C (optional)|
|Luminescence sensitivity||<10 amol ATP/well (96-well plate)|
|Crosstalk||3 x 10-5|
|Dynamic range||6 orders of magnitude|
|Interface||Windows software, connected to computer by USB|
|Operating system||Windows 7 or better|
|Available software packages||Simplicity for Raw Data included by default|
|Additional licenses available for Dual Assay, Fast Kinetics and Batch Protocol|
|Power Supply||110-240 V, 50/60 Hz, 30 VA (maximum 70 VA with 4 injectors and heating)|
|Temperature tolerance||Storage: 0-40° C|
|Operation: 15-30° C|
|Humidity tolerance||10-80% non-condensing|
|Dimensions (W x D x H)||385 x 410 x 255 mm|
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